Figure 3. Effect of downregulating p22phox on the activation of NOXs (NOX1, NOX2 and NOX4) and intracellular ROS generation in Ang II-stimulated ARPE-19 cells and hRPE.
(A) ARPE-19 cells were transfected with or without 100 nM P-1 in the presence or absence of 10−6 M Ang II for 48 h. Next, NOX1, NOX2 and NOX4 mRNA were detected with real-time PCR (*p < 0.05, **p < 0.01, n = 4–6). (B) hRPE were transfected with or without 100 nM P-1 in the presence or absence of 10−6 M Ang II for 48 h. The levels of NOX1, NOX2 and NOX4 mRNA were detected with real time PCR (*p < 0.05, **p < 0.01, ***p < 0.001, n = 4–6). (C) The ROS generation in each group of ARPE-19 cells and hRPE was analyzed by flow cytometry. The percentage of ROS generation is indicated on the ordinate and is normalized to the value of the control group (*p < 0.05, **p < 0.01, ***p < 0.001, n = 6–8). The data were shown as mean ± SEM.