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. 2015 Sep 29;5:14362. doi: 10.1038/srep14362

Figure 6. Involvement of the MAPK and NF-κB pathways in downregulating p22phox responded to the stimulation of Ang II.

Figure 6

(A) The protein expression of p38 MAPK, ERK1/2 and JNK, and their phosphorylation level (p-p38 MAPK, p-ERK1/2 and p-JNK) in the Ang II group compared with the P-1+ Ang II and untreated control groups were determined by Western blotting (*p < 0.05, **p < 0.01, n = 3–6). The band intensities of p-p38 MAPK, p-ERK1/2 and p-JNK were normalized to p38 MAPK, ERK1/2 and JNK, respectively. (B) The mRNA expression of IκBα, the inhibitor of NF-κB, in the Ang II group compared with the P-1+ Ang II and untreated control groups was detected by real-time PCR (***p < 0.001, n = 6). The protein expression of the NF-κB subunit p65 in the Ang II group compared with the P-1+ Ang II and untreated control groups was determined by Western blotting. Relative expression was normalized to β-actin (*p <0.05, **p <0.01, n = 3). The data were shown as mean ± SEM.