Figure 2.

Anti-tumor efficacy improved by efficient lymphoid draining and retention of nanoparticle-based cancer vaccines. (A) Lymphoid draining of a fluorophore-labeled pullulan nanogel 6 h post subcutaneous injection to mice. Scale bar, 1 mm. The nanogel loaded with a long peptide antigen (LPA) MAGE-A4 achieved better prophylactic (B) and therapeutic (C) efficacy compared to the soluble antigen; (B) Mice were immunized on day -7, followed by inoculation of tumor on day 0; (C) mice were inoculated with tumor on day 0, followed by immunization on day 4 and 11. Reproduced with permission [47].