Table A1.
UPLC conditions and peptide retention times.
| Gradient | Additional Features | Retention Time (min) | ||||
|---|---|---|---|---|---|---|
| Time (min) | Phase A | Phase B | Flux (mL/min) | 0.2 | 1338aa in dig. | 4.674 |
| 0 | 80 | 20 | Wavelength Abs (nm) | 280 | PADRE in dig. | 4.927 |
| 1.50 | 80 | 20 | Column Temp. (°C) | 35 °C | ||
| 1.51 | 55 | 45 | Filter time Constant | Normal | ||
| 3.00 | 80 | 20 | Volume injected (uL) | 5 | ||
1338aa in dig.: 1338aa dissolved in Chitosan/TPP/Poly (I:C) nanoparticle digested matrix; PADRE in dig.: PADRE dissolved in Chitosan/TPP/Poly (I:C) nanoparticle digested matrix.