Table 2.
Plasmids used in this study
| Plasmid name | Description | Source | Figure |
|---|---|---|---|
| pBlueScript SK+ GFP-PEP12::natNT2 | Amp | This study | Figure 2 |
| pRS416 TOR1 | CEN, URA3 | This study | Figure 4 |
| pRS413 | CEN, HIS3 | (Sikorski and Hieter, 1989) | Figure 4 |
| pRS315 HA-TOR1 | CEN, HIS3 | Gift from Dr Robbie Loewith | Figure 4 |
| pRS315 HA-tor1-D2275A | CEN, HIS3 | This study | Figure 4 |
| pRS315 HA-tor1-D2294E | CEN, HIS3 | This study | Figure 4 |
| pRS413 VAC17 | CEN, HIS3 | This study | Figure 4 |
| pRS413 TOR1 | CEN, HIS3 | This study | Figure 4 |
| pVT102-H | 2μ, HIS3 | (Vernet et al., 1987) | Figure 4 |
| pVT102-H SCH9 | 2μ, HIS3 | This study | Figure 4 |
| pVT102-H sch9-2D3E | 2μ, HIS3 | This study | Figure 4 |
| pVT102-H sch9-5A | 2μ, HIS3 | This study | Figure 4 |
| pRS416 GFP-SCH9 | CEN, URA3 | (Urban et al., 2007) | Figure 5 |
| pRS416 GFP-sch9-2D3E | CEN, URA3 | This study | Figure 5 |
| pRS416 TOR1-3xGFP(D330) | CEN, URA3 | This study | Figure 5 |
| pRS416 KOG1-3xGFP | CEN, URA3 | This study | Figure 5 |
| pRS416 FAB1-3xGFP | CEN, URA3 | (Jin et al., 2008) | Figure 5 |
| pRS413 | CEN, HIS3 | (Sikorski and Hieter, 1989) | Figure 2—figure supplement 1, Figure 4—figure supplement 1 |
| pRS413 MYO2 | CEN, HIS3 | (Catlett and Weisman, 1998) | Figure 2—figure supplement 1, Figure 4—figure supplement 1 |
| pRS413 myo2-N1304D | CEN, HIS3 | (Catlett et al., 2000) | Figure 2—figure supplement 1, Figure 4—figure supplement 1 |
| pRS416 KOG1 | CEN, URA3 | (Jin et al., 2014) | Figure 4—figure supplement 1 |
| pRS313 | CEN, HIS3 | (Sikorski and Hieter, 1989) | Figure 4—figure supplement 1 |
| pRS415 | CEN, LEU2 | (Sikorski and Hieter, 1989) | Figure 4—figure supplement 1 |
| pRS415 VAC17 | CEN, LEU2 | (Jin et al., 2009) | Figure 4—figure supplement 1 |
| pRS313 KOG1 | CEN, HIS3 | (Nakashima et al., 2008) | Figure 4—figure supplement 1 |
| pRS313 kog1-105 | CEN, HIS3 | (Nakashima et al., 2008) | Figure 4—figure supplement 1 |