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. 2015 Sep 29;5:14553. doi: 10.1038/srep14553

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Mice received intrathecally-administered C3 exoenzyme or Y27632, followed by LPS 30 min later. At 3 h after LPS injection, the 4–5th lumbar spinal cord was collected for (A) TNF-α, (B) IL-1β determination by ELISA. **P < 0.01 compared with NS control; ##P < 0.01 compared with LPS+saline group. n = 8 mice in each group.