Figure 1.

Depletion of Foxp3⁺CD4⁺CD25⁺ T cells from Balb/C mice after in vivo treatment with PC61 anti-CD25 mAb. Mice (n = 6 mice/group) were given a dose of 1 mg of PC 61 anti-CD25 monoclonal antibody i.p., and Foxp3⁺ CD4⁺CD25⁺ T cells (Treg cells) were monitored by flow cytometry at indicated time points. (A) Efficacy of Treg cell depletion. Plots were day 3 data following injection of anti-CD25 antibody, Foxp3⁺ CD4⁺CD25⁺ T cells were detected from peripheral blood (PBL), spleen (SPL), and draining lymph nodes (dLN). Results shown are representative of three experiments performed. (B) The distribution of Foxp3⁺ CD4⁺CD25⁺ T cells in naive mice. The percentages of Foxp3⁺ CD4⁺CD25⁺ T cells were detected from peripheral blood (PBL), spleen (SPL), and draining lymph nodes (dLN). Data are shown as mean ± SE. (C) Indicated time points show the kinetics of gradual replenishment of Foxp3⁺CD4⁺CD25⁺ T cells in PBL, SPL, and dLN following anti-CD25 injection. Data are shown as mean ± SD. Results are representative of two experiments performed.