Figure 3.

Increases the percentage of CD62L expression in P18 peptide-specific CD8⁺ T cells after Treg cells depletion in mice. Multiple lymph nodes including inguinal, mesenteric, and brachial obtained from groups of mice (n = 4 mice/group). (A) At day 0, Treg cells were depleted by administration of anti-CD25 antibody, and at day 3, plasmid gp120 DNA was injected. CD62L-specific tetramer-positive CD8⁺ T cells were measured. Differences in frequencies of tetramer in CD8 lymphocytes were statistically higher between 14 and 21 days (Student’s t-test with a cutoff of p = 0.05). (B) Mice at day 42 following gp120 DNA priming, then homologous gp120 DNA was boosted, the percentage of the gp120 antigen-specific H-2D^d/p18⁺CD62L⁺CD8⁺ T cells frequencies was monitored weekly, at 14 days following inoculation of DNA vaccine reached a maximum and was statistically higher in CD25 injected group (p < 0.01, Student’s t-test). Data are expressed as the mean percentage ± SE. Results shown are representative of three experiments performed.