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. 2015 Sep 21;7(9):8112–8126. doi: 10.3390/nu7095381

Figure 4.

Figure 4

DHA-derived 4-HHE reduces cell viability through apoptosis in VSMCs. (A) VSMCs were treated with a high concentration of DHA (150 μM), EPA (150 μM) or 4-HHE (50 μM) for 24 h. Cell viability was determined by the MTT assay. Values are expressed as percentage of cell survival, and each value represents the mean ± SE of five experiments (n = 15). (B) Cleaved caspase-3 and β-actin were determined by Western blotting. DHA (150 μM), EPA (150 μM) or 4-HHE (50 μM) were added for 6 h. * P <0.05, *** P < 0.001, compared with BSA control.