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. 2015 Sep 28;210(7):1085–1099. doi: 10.1083/jcb.201503006

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© 2015 Pacquelet et al.

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Figure 3. — ANI-2 accumulates and is required to misposition the furrow in cul-5 par-4 embryos. (A and B) ANI-2 strongly accumulates at the cortex between AB and P1 in cul-5(ok1706) par-4(it47) embryos. Confocal sections of two-cell embryos (A) of the indicated genotypes stained with anti-ANI-2 antibody and quantification (B) of ANI-2 cortical accumulation (see Materials and methods for details). Most wild-type embryos show no ANI-2 staining. ANI-2 weakly accumulates in cul-5(ok1706) and par-4(it47) embryos (arrowheads) and strongly in cul-5(ok1706) par-4(it47) embryos. Bars, 10 µm. See also Fig. S1 D. (C) ani-2 RNAi suppresses furrow position defects of cul-5(ok1706) par-4(it47) embryos. Furrow position was measured when the furrow spanned the entire embryo. P-values from Student’s t test.