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. 2015 Sep 28;210(7):1117–1131. doi: 10.1083/jcb.201504007

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© 2015 Grimsey et al.

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Figure 1. — Activated PAR1 is modified with K63-linked ubiquitin. (A) Ubiquitination of endogenous PAR1 detected in endothelial cells after stimulation with 10 nM α-Th. (B) Ubiquitination of FLAG-tagged PAR1 WT expressed in HeLa cells after stimulation with 100 µM SFLLRN. (C) Poly K63- or K48-linked ubiquitin chains (300 ng each) detected with anti-pan ubiquitin (Ub) P4D1 or anti-K63 Ub specific antibodies. (D) Ubiquitination of FLAG-tagged PAR1 in HeLa cells after stimulation with 100 µM SFLLRN. See Fig. S1 A.