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. 2015 Sep 28;210(7):1117–1131. doi: 10.1083/jcb.201504007

Figure 2.

Figure 2.

Ubiquitination of PAR1 is required for p38 activation. (A and B) HeLa cells expressing FLAG-PAR1 WT or ubiquitin-deficient (0K) mutant were stimulated with 10 nM α-Th, and p38, MSK-1, and ERK1/2 phosphorylation was determined. The data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test (*, P < 0.05). See Fig. S1 (B–D). (C) PAR1 WT and 0K HeLa cells were incubated with 10 nM α-Th, and p38 kinase activity was determined in vitro. The data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test (**, P < 0.01). (D) HeLa cells expressing PAR1 WT or 0K were stimulated with 10 nM α-Th or 16 nM TNF, and p38 phosphorylation was determined. The data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test (***, P < 0.001).