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. 2015 Sep 28;210(7):1117–1131. doi: 10.1083/jcb.201504007

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© 2015 Grimsey et al.

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Figure 4. — Thrombin-induced p38 activation requires NEDD4-2 expression. (A) PAR1-expressing HeLa cells transfected with ns or NEDD4-2 siRNA were stimulated with 10 nM α-Th, and phosphorylation of p38, MSK1, and ERK1/2 was determined. The data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test (*, P < 0.05; **, P < 0.01). See Fig. S2 (D–F). (B) Endothelial cells were transfected with ns or NEDD4-2 siRNAs and incubated with 10 nM α-Th. Phosphorylation of p38 and MSK1 was then determined. The data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test (*, P < 0.05; **, P < 0.01). (C) PAR1 HeLa cells transfected with ns or NEDD4-2 (N4-2) siRNAs were treated with 16 nM TNF, and phosphorylation of p38 and MSK1 was determined. The data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test.