Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 28;210(7):1117–1131. doi: 10.1083/jcb.201504007

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Grimsey et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 9. — P2Y₁ receptor-induced p38 activation is mediated by a ubiquitin- and TAB1–TAB2-dependent pathway. (A) HA-tagged P2Y₁ WT or K3R mutant expressed in HeLa cells were stimulated with 10 µM ADP and ubiquitination was detected. See Fig. S5 (A–C). (B) HA-P2Y₁ WT or K3R HeLa cells were stimulated with 10 µM ADP, and p38, MSK1, and ERK1/2 phosphorylation was detected. The data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test (*, P < 0.05; **, P < 0.01). (C) HA-P2Y₁ HeLa cells transfected with ns or NEDD4-2 siRNA were treated with 10 µM ADP, and p38, MSK1, and ERK1/2 phosphorylation was detected. The data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test (*, P < 0.05; **, P < 0.01). See Fig. S5 (D and E). (D) HA-P2Y₁ HeLa cells pretreated with DMSO or 5 µM SB203580 for 20 min were stimulated with 10 µM ADP, and p38, MSK1, and ERK1/2 phosphorylation was detected. Data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test (*, P < 0.05; **, P < 0.01). (E) HA-P2Y₁ HeLa cells transfected with ns or TAB1 and TAB2 siRNA were stimulated with 10 µM ADP, and p38 and MSK1 phosphorylation was detected. The data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test (**, P < 0.01; ***, P < 0.001). See Fig. S5 F. (F) HA-P2Y₁ HeLa cells were stimulated with 10 µM ADP, and TAB1 expression was detected. The data (mean ± SD [error bars], n = 3) were analyzed using a Student’s t test (***, P < 0.001).