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. 2015 Sep 28;210(7):1257–1268. doi: 10.1083/jcb.201502035

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© 2015 Chetty et al.

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Figure 1. — PP1 treatment improves the differentiation capacity of hPSCs in a dose-dependent manner. (A) Chemical structure of 4-amino-5-(4-methylphenyl)-7-(t-butyl)pyrazolo-d-3,4-pyrimidine (PP1). (B) Schematic of directed differentiation of hPSCs into Brachy+ cells after no treatment (control) or a 24-h PP1 treatment at varying doses. (C–E) Percentages (C) and absolute numbers (D) of control and PP1-treated HUES6 hPSCs differentiating into Brachy+ cells in relation to the total cell numbers (E) in control and PP1-treated cultures. (F) Immunostaining for Oct4, Brachy, and DAPI in control and PP1-treated cultures after directed differentiation. Error bars indicate SEM of three replicates. Bars, 200 µM.