Skip to main content
. 2015 Sep 9;112(38):E5351–E5360. doi: 10.1073/pnas.1515982112

Fig. 6.

Fig. 6.

Detection of social deficits in BTBR animals. C57BL/6N or BTBR animals were tested with a BALB/c male in an unfamiliar, neutral cage. (A) Raster plots showing the supervised classifier-based machine annotations of social investigation behavior exhibited by C57BL/6N or BTBR tester mice in the first 5 min of their interactions with BALB/c target animals. (B) Histograms of behavioral bout duration (fraction of total time) for social investigation exhibited by C57BL/6N or BTBR animals toward BALB/c during the first 5 min of their interactions. (C) Percentage of time spent on close investigation during the same behavior sessions. (D) Distribution of the distance between centroid locations of two interacting animals (fraction of total time) during the same behavior sessions. (E) Percentage of time the centroids of two interacting animals are within 6 cm during the same behavior sessions. (F) Distribution of the distance between the front end of the subject (BTBR or C57BL/6N) and the centroid of the BALB/c animal (fraction of total time) during the same behavior sessions. Note that no significant difference between tester strains is evident using this tracker-based approach to analyze the interactions. (G) Percentage of time the front end of the tester (BTBR or C57BL/6N) mouse is within 4 cm from the centroid of the target BALB/c animals during the same behavior sessions. Metrics in D and E are based solely on output from the tracker, metrics in F and G are based on output from the tracker and pose estimator, and metrics in A–C are derived from the automated behavioral classifier. See Fig. S6 for metrics equivalent to D–G analyzed for the BALB/c target mouse.