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. 2015 Sep 8;112(38):11876–11880. doi: 10.1073/pnas.1509929112

Fig. 3.

Fig. 3.

Rapid and transient Fas-mediated palmitoylation of Lck. (A) Palmitoylation of Lck in the presence of Fas ligand (Upper) and input representing 5% of total protein extracts (Lower). Jurkat cells were incubated with 1 µM 17-ODA or DMSO at room temperature for 30 min. Fas ligand was added during incubation with 17-ODA for the indicated times. (B) Fas-mediated palmitoylation of Lck in J.gamma1 (PLC-γ1 null) Jurkat cells. (C) Fas-mediated palmitoylation of Lck in the presence of APT1 inhibitor palmostatin B. Jurkat cells were pretreated with 10 µM palmostatin B or DMSO for 30 min before the addition of 1 µM 17-ODA and Fas ligand.