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MβCD inhibits Fas-mediated PLC-γ1 activation and calcium release. (A) Jurkat cells preincubated with 5 mM and 10 mM MβCD for 30 min and treated with Fas ligand for 0, 1, and 10 min. Total cell lysates were analyzed by Western blot analysis. (B) Jurkat cells pretreated for 5 min with MβCD. Intracellular calcium release was determined with Fura-2 imaging.
