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Effect of extracellular (BAPTA) and intracellular (BAPTA-AM) calcium chelators on Fas-mediated Lck palmitoylation. Control Jurkat cells (Top) or Jurkat cells preincubated with 5 mM BAPTA (Middle) or 10 µM BAPTA-AM (Bottom) for 30 min at room temperature were stimulated with Fas ligand in the presence of 17-ODA. Both BAPTA and BAPTA-AM inhibited FasL-dependent palmitoylation.
