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. 2015 Aug 31;112(38):E5281–E5289. doi: 10.1073/pnas.1511270112

Fig. 1.

Fig. 1.

BCR repertoire and mutation analysis of human PB memory B-cell subsets. (A) The relative use of individual IGHV gene segments of families 1, 3, 4, and 7 among memory B-cell subpopulations shows highly similar patterns. Statistically significant differences between individual subsets are marked (*P < 0.05, **P < 0.01, ***P < 0.001; Fisher’s exact test). Test results were not corrected for multiple comparisons to estimate the maximum number of gene segments differing between B-cell subsets with the full specificity of Fisher's exact test. Only IGHV gene segments comprising at least 5% of total sequences in at least one condition and showing at least twofold difference in frequency between two B-cell subsets were considered. The separator in each column marks the amount of sequences contributed by each vial. (B) IGHV gene mutation frequencies (mutations/100 bp) of memory B-cell subsets are distinct. Median values (black bars) are given as numbers, and box plots represent 25 and 75 percentiles. ***P < 0.001; t test.