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. 2015 Aug 31;112(38):E5281–E5289. doi: 10.1073/pnas.1511270112

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Fig. S1. — Cell sorting strategy of memory B-cell subpopulations. (A) IgM⁺IgD⁺CD27⁺ and IgM-only B cells are separated after enrichment of B cells by CD19 MACS according to their surface IgD level. CD27^high plasmablasts are excluded. (B) Postsort analysis of IgM⁺IgD⁺CD27⁺ and IgM-only B cells from donor 1. (C) IgG⁺CD27⁺ and IgG⁺ CD27⁻ B cells are defined by surface IgG and CD27 expression. Pre- and postsort analysis of donor 1 is shown. (D) The relative use of individual IGHV gene segments of families 1, 3, 4, and 7 among memory B-cell subpopulations and biological replicates is highly similar. Minor variations between subpopulations are detectable at similar ranges in biological replicates. No statistically significant differences are detectable between any two conditions among single IGHV genes with >5% frequency and greater than twofold change. The separator in each column marks the amount of sequences contributed by each allelic variant of the respective IGHV gene. (E) CDRIII length spectratyping reveals highly similar distributions between memory B-cell subpopulations. The separator in each column marks the amount of sequences contributed by each vial.