Table S1.
Input cell numbers and number of sequences retrieved by NGS
| Cell sample (replicate vial) | Donor 1 | ||||||||
| IgG+CD27− | IgG+CD27+ | IgM+IgD+CD27+ | IgM-only CD27+ | Total | |||||
| A | B | A | B | A | B | A | B | A+B | |
| # input cells | 8,000 | 8,000 | 45,000 | 45,000 | 50,000 | 50,000 | 5,500 | 5,500 | 217,000 |
| # 454 reads retrieved | 75,747 | 77,171 | 363,212 | 392,155 | 582,051 | 340,066 | 85,455 | 75,382 | 1,991,239 |
| # reads> 300 bp length | 73,841 | 75,112 | 320,145 | 365,358 | 577,016 | 331,317 | 83,696 | 74,287 | 1,900,772 |
| # unique sequences (identical sequences collapsed) | 28,677 | 26,567 | 79,599 | 78,315 | 148,274 | 81,009 | 22,525 | 23,078 | 488,044 |
| # sequences with ≥ twofold coverage | 7,710 | 6,949 | 21,155 | 20,271 | 30,285 | 15,807 | 5,366 | 5,076 | 112,619 |
| # sequences after 454 error correction* | 4,766 | 3,992 | 12,121 | 9,497 | 19,410 | 9,077 | 3,889 | 3,900 | 66,652 |
| Donor 2 | |||||||||
| C | D | C | D | C | D | C | D | C+D | |
| # input cells | 10,000 | 10,000 | 46,000 | 46,000 | 50,000 | 50,000 | 5,500 | 5,500 | 223,000 |
| # 454 reads retrieved | 79,630 | 93,677 | 360,117 | 519,422 | 547,384 | 409,085 | 67,107 | 52,783 | 2,129,205 |
| # reads> 300 bp length | 68,466 | 86,169 | 274,638 | 387,728 | 456,433 | 344,669 | 58,871 | 45,839 | 1,722,813 |
| # unique sequences (collapsing of identical sequences) | 39,313 | 49,170 | 103,627 | 115,420 | 235,542 | 183,475 | 29,655 | 24,712 | 780,914 |
| # sequences with ≥ twofold coverage | 4,549 | 6,038 | 13,534 | 16,068 | 21,179 | 16,206 | 2,750 | 2,126 | 82,450 |
| # sequences after 454 error correction* | 2,372 | 2,584 | 6,138 | 4,637 | 11,683 | 10,219 | 1,705 | 1,320 | 40,658 |
*
Insertions/deletions arising from incorrect length determination of sequences of identical nucleotides, typically occurring by 454 base calling.