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. 2015 Sep 8;112(38):11959–11964. doi: 10.1073/pnas.1500525112

Fig. 1.

Fig. 1.

Effects of isoflurane on synaptic vesicle recycling pool size and exocytosis kinetics. (A) Synaptic vesicle exocytosis kinetics were determined by fitting vGlut-pHluorin fluorescence traces during prolonged action potential (AP) stimulation in the presence of bafilomycin A1 (10 Hz for 150 s; 1,500 APs) for control or 0.7 ± 0.06 mM isoflurane [twice the minimum alveolar concentration (MAC); equivalent to EC50 for immobilization in rat] in the presence of 2 mM extracellular Ca2+ ([Ca2+]e). (B) (Upper) Effect of isoflurane on mean exocytosis time constant. Traces were fit with a single exponential function to determine the time constant for exocytosis of the recycling pool. **P < 0.01 by two-tailed unpaired t test. (Lower) Effect of isoflurane on total recycling pool (TRP) size as a fraction of total pool size (determined using 50 mM NH4Cl), P = 0.21 by two-tailed unpaired t test. n = 12.