Fig. S5.

Gating strategy and analysis of ThEO/TcEO cells in the spleens and draining lymph nodes of tumor-bearing mice. (A) Representative FACS plots gating on Eomes⁺KLRG1⁺ CD4 T cells (Top) or CD8 T cells (Bottom) of mice receiving various immunomodulatory antibody therapies alone or in combination with the HPV peptide vaccine. Quantitation of Eomes⁺KLRG1⁺ ThEO (Left) and TcEO (Right) cells in the spleen (B) and draining lymph nodes (C) of tumor-bearing mice. Experiments were performed in duplicate by pooling spleens or lymph nodes from at least three mice per group. Bars represent mean ± SD. Statistical significance was calculated using a one-way ANOVA.