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4-1BB expression on NK T cells is induced by αGalCer. Naïve splenocytes were cultured in complete RPMI with 2 ng/mL αGalCer for 48 h with or without 40 μg/mL anti–4-1BB (clone LOB12.3). Cells were stained for 4-1BB expression using 4-1BB-PE (clone 17B5) and for NK T cells and analyzed by flow cytometry. The mean fluorescence intensity (MFI) of 4-1BB expression in each NK T-cell population is shown.
