Table S2.
Details of primers used, their source, annealing temperature, and expected amplicon size
Target | Name | Sequence (5′–3′) | Reference or source | Annealing | Amplicon size, bp |
E. tenella | TEN-F | TCGTCTTTGGCTGGCTATTC | Vrba et al., 2010 (63) | 56 °C | 100 |
gDNA, diagnostic | TEN-R | CAGAGAGTCGCCGTCACAGT | Vrba et al., 2010 (63) | ||
Eimeria 5S rRNA | 5S_For | TCATCACCCAAAGGGATT | Blake et al., 2006 (64) | 56 °C | ∼110 |
5S_Rev | TTCATACTGCGTCTAATGCAC | Blake et al., 2006 (64) | |||
E. tenella AMA-1 | EtAMA1_fl_F | CACCATGCGGCGGCTTTC | This study | 60 °C | 1,610* |
cDNA | EtAMA1_fl_R | CCTGGTCCAGCAGCACTTGG | This study |
Amplicon size calculated using cDNA as template.