Table 3.

The impact of parental ratio during E. tenella coinfection on the likelihood of cross-fertilization (36 sporocysts per ratio)

Houghton:Nt2 ratio	No. birds	Sporocysts per bird	Self-fertilization			Cross-fertilization
			Expected (Houghton or Nt2)	Observed Houghton	Observed Nt2	Expected	Observed Houghton or Nt2
1:1	2	18	18	8	5	18	23
2:1	2	18	20	11	7	16	18
5:1	2	18	24	20	2	12	14
10:1	2	18	30	24	2	6	10

Birds were coinfected with Houghton and Nt2 E. tenella strains. Single-progeny sporocysts were identified as the product of self- or cross-fertilization following FACS purification and whole-genome amplification using Sequenom MassARRAY and comparison with both parental genotypes.