Figure 5. The comparative analysis of CO genes between Brassicaceae and Grass species.

(a) The multiple sequence alignment of CO genes. The orange solid rectangle represents the five Brassicaceae species, and the green solid rectangle represents the three Grass species. The red hollow rectangle indicates the family-specific amino acids. (b) The phylogenetic trees were constructed using the CO protein and gene sequences. The numbers on the branches of the phylogenetic tree represent the bootstrap supports provided by the NJ, ME and ML methods. (c) The genetic distance in Brassicaceae and Grass was estimated using the CO protein and gene sequences.