Figure 1. Schematic representation of the experimental procedure used for the redox arrays.

THP-1 cells were incubated with cell-permeable BioGEE to label the intracellular pool of proteins. After 24 hour incubation with LPS to allow secretion of proteins, cell conditioned medium and cell lysates were collected. After removal of free BioGEE, proteins were applied to the antibody membrane-based array. Biotin-labelled proteins were detected by the addition of streptavidin-HRP, followed by chemiluminescent detection.