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. 2015 Sep 29;5:14630. doi: 10.1038/srep14630

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(a) Western blotting of recombinant IL-1 sRII treated with BioGEE in vitro probed with anti-IL-1 sRII antibody (left panel) or streptavidin-HRP (right panel). The signal from the streptavidin-labelled protein is lost upon treatment with DTT. (b) Immunoprecipitation of IL-1 sRII from cell culture media of untreated THP-1 cells preincubated with BioGEE. Biotin labelling of the IL-1 sRII was confirmed by stripping of the membrane and re-probing with streptavidin-HRP. (c) Release of IL-1 sRII from THP-1 cells in response to treatment with LPS.