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. 2015 Sep 29;6:496. doi: 10.3389/fimmu.2015.00496

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Copyright © 2015 Soto, Ferrier, Aravena, Fonseca, Berendsen, Biere, Bueno, Ramos, Aguillón and Catalán.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Frequencies of B-cell subpopulations in systemic sclerosis patients. (A) Flow cytometry gating strategy to identify transitional B cells (CD19⁺CD24^highCD38^high), naive B cells (CD19⁺CD24^intCD38^int), and memory B cells (CD19⁺CD24^highCD38⁻). (B) Percentage of CD19⁺ B cells within peripheral blood mononuclear cells (PBMC) in healthy controls (HC) (n = 24) and systemic sclerosis (SSc) patients (n = 24). (C) Percentage of transitional, naive, or memory B-cell subpopulations within total CD19⁺ B cells in HC (white circles) (n = 31) and SSc patients (gray circles) (n = 30). *P < 0.05, ***P < 0.001, Mann–Whitney U test.