Protein blots of EV/HPs, siRNA-SNAILEV/HPs, HIV/HPs and siRNA-SNAIL-HIV/HPs were probed for SNAIL and the same blots were reprobed for VDR and actin (n=3). Representative gels are displayed.
Cumulative densitometry data of the gels displayed in B are shown. *P<0.05 compared to siRNA-SNAIL/HIV/HP
RNAs were extracted from EV/HPs and siRNASNAIL/EV/HPs. cDNA were amplified with VDR specific primer and mRNA was quantified by real time PCR. Cumulative data (n=3) are displayed in bar graphs.
Protein blots of C/293T and SNAIL/293T cells were probed for SNAIL and the same blots were reprobed for VDR and actin (n=3). Gels from three different samples are displayed.
Cumulative densitometric data on VDR/actin ratios of gels shown in E. *P<0.01 vs C/293T.
Cumulative densitometric data on SNAIL/actin ratios of gels shown in E are displayed as a bar diagram (n=3). *P<0.01 vs C/293T.
RNA was extracted from control and SNAIL/293T cells and cDNAs were amplified with VDR specific primer and mRNA was quantified by real time PCR. Cumulative data (n=3) are displayed in bar graphs. *P<0.05