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. Author manuscript; available in PMC: 2016 Oct 9.
Published in final edited form as: J Mol Biol. 2015 Jul 22;427(20):3201–3215. doi: 10.1016/j.jmb.2015.07.011

Fig. 5. HIV enhances podocyte DNA methyl transferase production and increases VDR methylation.

Fig. 5

  1. Protein blots of EV/HPS (control) and HIV/HP (HIV) were probed for DNMT3b and reprobed for actin (n=7). Gels from two different lysates are displayed. Cumulative data (n=7) are shown in bar graphs.
  2. DNA was extracted from EV/HPs and HIV/HPs and CpG DNA methylation qPCR and methylation qPCR Assay with VDR specific primers were carried out. Methylated fraction was calculated by measuring unmethylated, hypermethylated and resistance to get methylated fractions. Cumulative data are shown in bar graphs. *P<0.05 vs. EV.