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. Author manuscript; available in PMC: 2016 Oct 9.
Published in final edited form as: J Mol Biol. 2015 Jul 22;427(20):3201–3215. doi: 10.1016/j.jmb.2015.07.011

Fig.6. Both demethylating agents and deacetylating agents (HDAC inhibitors) augment VDA-induced podocyte VDR upregulation in HIV milieu.

Fig.6

  1. EV/HPs and HIV/HPs were incubated in media containing either buffer, VDA (1 nM) for 4 h followed by treatment with or without azacytidine (DAC, 5 μM) for 48 hours at 33°C (n=3). Protein blots were probed for VDR and reprobed for actin. Representative gels are displayed. Cumulative data are shown in bar graphs. *P<0.05 compared to all other variables; ≠P<0.05 compared to HIV alone; #P<compared to EV.
  2. EV/HPs and HIV/HPs were pretreated with either buffer or HDACI (SAHA, 10 μM) for 2h followed incubation in media containing either buffer, VDA (1 nM) in the presence or absence of SAHA for 48 hours (n=3). Protein blots were probed for VDR and reprobed for actin. Representative gels are displayed. Cumulative data are shown in a bar diagram. *P<0.05 compared to EV; ≠P<0.05 compared to HIV alone; #P<0.05 compared to EV.