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. 2015 Sep 29;6:808. doi: 10.3389/fpls.2015.00808

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Copyright © 2015 Peng, Jia and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transformation, regeneration, and characterization of trifoliate orange transgenic plants. (A,B) Culture of the stem segments on selection medium for 30 d (A) and 60 d (B), respectively. (C) Regeneration of kanamycin -resistant shoots on the selection medium. (D) A rooting plant on the root-inducing medium. (E) Genomic PCR of the kanamycin-resistant plants using designed primers specific to PtrPRP (upper) and NPTII (bottom), respectively. (F) Expression analysis of PtrPRP in six positive transgenic plants, as revealed by RT-PCR. Actin gene was used as an internal control. (G) Analysis of PtrPRP expression level in RNAi-51 using qRT-PCR.