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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Aug 8;1850(11):2318–2328. doi: 10.1016/j.bbagen.2015.08.002

Fig. 3. SypHer-2 fused with Homer and Synaptophysin allows pH detection in synaptic structures.

Fig. 3

A) Expression of SypHer-2-Homer and Synaptophysin-SypHer-2 in cultured mouse hippocampal neurons; B) pH oscillations registered in a single postsynaptic terminus using the SypHer-2-Homer; C) pH oscillations registered in a single presynaptic bouton using Synaptophysin-SypHer-2; D) amplitude of pH oscillations in presynaptic vs. postsynaptic termini. All experiments were replicated with 3 to 5 neuronal cells from two different preparations. Scale bars are 40 μm.