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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Neurochem Int. 2015 Aug 10;89:243–248. doi: 10.1016/j.neuint.2015.08.003

Figure 5. Time course of fisetin accumulation in the brains of living mice following intraperitoneal injection.

Figure 5

Fisetin (74 mg/kg) was injected intraperitoneally and the characteristic green fluorescence of fisetin (max at 550 nm) was detected in the blood vessels within 8 min after injection (b) and in both the blood vessels and brain parenchyma 15 min and 25 min after injection (c, d). No detectable levels of green emission were seen in prior to fisetin injection (a). Fisetin progressively moves out of the blood vessels into the parenchyma following intraperitoneal injection (e, f). Spectra were acquired from regions of interest (ROI) covering blood vessels (red), adjacent areas of parenchyma (green) and more distant areas (blue, used for background correction). Spectra were acquired before (1) and at 8 min (2), 15 min (3) and 25 min (4) after intraperitoneal injection on ROIs covering blood vessels (e) and adjacent parenchyma (f). FOV was 225 μm × 225 μm in all experiments.