Table I. Reagents.
| siRNA | Vendor | Catalog # | Antibody | Vendor | Cat # | Dilution |
|---|---|---|---|---|---|---|
| AllStars neg ctrl siRNA | Qiagen | 1027281 | p110α | Cell Signaling | 4249 | 1:1,000 (immunoblot) |
| Universal neg ctrl siRNA | Sigma | SIC001 | P-S6 (S235/236) | Cell Signaling | 4858 | 1:1,000 (immunoblot) |
| EphA2 siRNA #6 | Qiagen | SI00300188 | S6 | Cell Signaling | 2317 | 1:1,000 (immunoblot) |
| EphA2 siRNA #12 | Qiagen | SI04434990 | P-Akt (S473) | Cell Signaling | 9271 | 1:1,000 (immunoblot) |
| TGM2 siRNA #1 | Qiagen | SI00743715 | Akt | Cell Signaling | 9272 | 1:2,000 (immunoblot) |
| TGM2 siRNA #5 | Qiagen | SI03020927 | P-ERK (T202/Y204) | Cell Signaling | 4370 | 1:1,000 (immunoblot) |
| TGM2 siRNA #6 | Qiagen | SI03055465 | ERK | Cell Signaling | 9102 | 1:2,000 (immunoblot) |
| TGM2 siRNA #7 | Qiagen | SI03101966 | Laminin α3 | R&D Systems | MAB2144 | 10 μg/ml (immunofluor) |
| PXDN siRNA | Sigma | SASI_Hs02_00344041 | Laminin β3 | Epitomics | 7030 | 1:1,000 (immunoblot) |
| PXDN siRNA | Sigma | SASI_Hs02_00344042 | Laminin β3 | Santa Cruz | 133178 | 1:500 (immunoblot) |
| LAMC2 siRNA | Sigma | SASI_Hs01_00136952 | Laminin γ2 | Millipore | MAB19562 | 1:1,000 (immunoblot) |
| LAMC2 siRNA | Sigma | SASI_Hs01_00136953 | Fibronectin | Epitomics | 1574 | 1:2,000 (immunoblot) |
| LAMB3 siRNA | Sigma | SASI_Hs01_00247331 | THBS1 | Millipore | BA24 | 1 μg/ml (immunoblot) |
| LAMB3 siRNA | Sigma | SASI_Hs01_00247332 | β-Actin | Sigma | A2228 | 1:10,000 (immunoblot) |
| ITGA5 siRNA | Sigma | SASI_Hs01_00058581 | EphA2 | Millipore | 05–480 | 1:500 (immunoblot) |
| ITGA5 siRNA | Sigma | SASI_Hs02_00333426 | TGM2 | Thermo | Ms-300 | 1:500 (immunoblot) |
| THBS1 siRNA | Sigma | SASI_Hs01_00148022 | Integrin α2 | Santa Cruz | 6585 | 1:500 (immunoblot) |
| THBS1 siRNA | Sigma | SASI_Hs01_00148023 | Integrin α5 | Cell Signaling | 4705 | 1:1,000 (immunoblot) |
| AREG siRNA | Sigma | SASI_Hs01_00014263 | Integrin α6 | Cell Signaling | 3750 | 1:1,000 (immunoblot) |
| AREG siRNA | Sigma | SASI_Hs01_00332258 | Integrin β1 | Cell Signaling | 4706 | 1:1,000 (immunoblot) |
| Integrin β4 | Cell Signaling | 4707 | 1:1,000 (immunoblot) | |||
| Vimentin | Cell Signaling | 5741 | 1:1,000 (immunoblot) | |||
| PTPRF | R&D Systems | MAB3004 | 0.5 μg/ml (immunoblot) | |||
| shRNA | Vendor | Catalog # | CAB39 | Cell Signaling | 2716 | 1:1,000 (immunoblot) |
| TGM2 IPTG induc shRNA | Sigma | TRCN0000272817 | Gelsolin | Cell Signaling | 8090 | 1:1,000 (immunoblot) |
| PTPRFshRNA | Sigma | TRCN0000002855 | P-Rb (S780) | Cell Signaling | 9307 | 1:1,000 (immunoblot) |
| PTPRFshRNA | Sigma | TRCN0000002857 | P-EGFR (Y1173) | Santa Cruz | 12351 | 1:500 (immunoblot) |
| non-silencing ctrl shRNA | Sigma | SHC002 | P-EGFR (Y1068) | Cell Signaling | 3777 | 1:1,000 (immunoblot) |
| P-EGFR (Y1148) | Cell Signaling | 4404 | 1:1,000 (immunoblot) | |||
| EGFR | Santa Cruz | 03 | 1:500 (immunoblot) | |||
| HER2 | Thermo | Rb103 | 1:1,000 (immunoblot) | |||
| P-Tyr | R&D Systems | HAM1676 | 1:5,000 (immunoblot) | |||
| AREG | R&D Systems | AF262 | 0.5 μg/ml (immunoblot) | |||
| AREG | R&D Systems | MAB262 | 25 μg/ml (neutralize) | |||
| mouse IgG | R&D Systems | MAB002 | 25 μg/ml (neutralize) | |||