Table III. Top targets related to lymph node metastasis in grade 1 breast tumors and their clinicopathological selectivity. Proteins with low number of observations (N ≤ 10) in the proteomics study are in italics (see Experimental procedures for details). Significant changes in protein levels and expression (according to criteria defined in the Experimental Procedures section) are indicated in bold.

		G1: N1–2/N0		G3:N1–2/N0		N1–2/N0		G3/G1		ER+/ER-		HER2+/HER2-
		fold change (IHC trend)	p value	fold change (IHC trend)	p value	fold change (IHC trend)	p value	fold change (IHC trend)	p value	fold change (IHC trend)	p value	fold change (IHC trend)	p value
CPB1	protein	4.34	0.002	0.87	0.741	1.95	0.114	0.55	0.063	2.22	0.007	0.56	0.248
	transcript	6.737	0.025	0.197	0.073	1.199	0.779	0.192	0.009	15.571	0.001	1.266	0.799
	IHC total intensity	↑	0.096	∼	0.514	∼	0.607	∼	0.206	↑	0.514	∼	0.722
	IHC histoscore	↑	0.039	∼	0.475	∼	0.406	∼	0.246	0.449	∼	0.589
RNF25	protein	1.44	0.053	1.18	0.354	1.30	0.089	0.84	0.379	N<3	N<3	N<3	N<3
	transcript	1.267	0.002	0.870	0.218	1.050	0.512	0.763	0.000	1.418	0.002	1.168	0.160
	IHC tumor cells staining	∼	0.817	∼	0.766	↑	0.570	∼	0.626	↓	0.286	↑	0.825
	IHC stromal cell staining	∼	1.000	↑	0.742	∼	0.905	↓	0.521	↓	0.439	↓	0.433
PDLIM2	protein	1.30	0.009	1.06	0.866	1.18	0.415	0.71	0.008	1.42	0.208	0.65	0.060
	transcript	1.287	0.007	0.851	0.215	1.047	0.571	1.038	0.645	1.159	0.258	1.056	0.671
STMN1	protein	1.24	0.002	1.02	0.720	1.12	0.012	1.14	0.039	1.01	0.949	0.77	0.036
	transcript	1.283	0.050	1.116	0.608	1.197	0.211	2.042	0.000	1.250	0.296	1.086	0.705
	IHC total intensity	↑	0.454	↓	0.879	↑	0.750	↑	0.006	∼	0.879	∼	0.110
	IHC % of stained cells	∼	0.415	∼	0.350	∼	0.327	↑	0.000	∼	0.73	∼	0.246
	IHC membrane staining	↑	0.108	↓	0.370	∼	1.000	↑	0.000	∼	0.562	↓	0.210
	IHC stained lymphocytes	∼	1.000	↓	0.659	↓	0.434	↑	0.203	↓	0.05	↓	1.000
TMSB10	protein	1.32	0.003	0.97	0.668	1.13	0.066	1.26	0.005	0.89	0.161	1.09	0.540
	transcript	1.245	0.050	0.990	0.951	1.110	0.364	1.727	0.000	0.738	0.066	1.925	0.000
RELA	protein	1.30	0.020	1.10	0.246	1.20	0.007	0.85	0.029	1.26	0.161	0.80	0.066
	transcript	1.215	0.012	1.020	0.835	1.111	0.850	1.097	0.133	1.094	0.337	1.050	0.601
YWHAH	protein	1.20	0.012	1.04	0.382	1.12	0.007	0.84	0.000	1.17	0.022	0.83	0.001
	transcript	1.200	0.024	0.877	0.330	1.026	0.750	1.226	0.011	1.056	0.687	1.075	0.583
	IHC tumor cells staining	∼	1.000	∼	0.145	∼	0.348	↑	0.002	↓	0.336	∼	0.767
	IHC membrane staining in tumor cells	↓	0.246	↓	0.552	↓	0.269	↑	0.060	↓	0.126	∼	1.000
	IHC stromal cell staining	∼	0.866	∼	0.789	∼	0.788	↑	0.070	∼	0.529	∼	0.840
TRAF3IP2	protein	1.24	0.387	1.52	0.001	1.37	0.002	0.63	0.000	1.32	0.042	1.09	0.403
	transcript	1.404	0.003	1.084	0.505	1.233	0.012	0.858	0.070	1.088	0.486	1.335	0.023
	IHC percentage of stained cells	∼	0.928	∼	0.220	∼	0.357	∼	0.494	∼	0.403	∼	0.342
	IHC stromal fibroblasts stained	∼	1.000	↑	0.755	∼	0.825	∼	0.825	↑	0.063	↓	0.191