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. 2015 Apr 17;14(7):2030–2041. doi: 10.1074/mcp.O115.049460

Fig. 3.

Fig. 3.

Workflow of the high-throughput LC-MS/MS protein interaction analysis pipeline. Both culturing of yeast cells and affinity purification are performed in 96-well plate format, thus parallelizing sample preparation and minimizing handling errors. LC-MS/MS analysis of 96 pull-down samples in 1 day is achieved through a double-barrel chromatography setup and the increased sequencing speed of the Q Exactive HF mass spectrometer.