Table I. Example of Cx43-interacting proteins likely related to Cx trafficking, GJ assembly, GJIC, and turnover (chondrocytes from healthy donors). The cytoskeletal proteins that interact or colocalize with Cxs have a critical functional importance in Cx trafficking and assembly of Cx into GJs. Microfilaments and microtubules are involved in turnover mechanisms that compromise the control of GJIC. The values of peptide probability or score, sequence coverage and number of peptides found are shown in supplementary Fig. S1. Only proteins that show >95% confidence and score >1.3 were considered for this study.
| Protein Group\Name | Gene name |
|---|---|
| α-a and ß-tubulinab | TUBA1B\TUBB\TUBB2A\ TUBB2C |
| α-actina and actin related proteins | ACTB\ACTR3B\ACTN4 |
| Translocon-associated protein subunit delta | SSR4 |
| Myosin motor proteinsa (Myosin ICb) | MYL12B\MYH9\MYO1B |
| Vimentina | VIM |
| Vinculinab | VCL |
| Talin-1b | TLN1 |
| Filamin A, alpha | FLNA |
| Dihydropyrimidinase-related protein 2 | DPYSL2 |
| SEC22 vesicle trafficking protein homolog B | SEC22B |
| Gelsolin | GSN |
| Profilin-1 | PFN1 |
| Erzinab | EZR |
| Radixinb | RDX |
| Moesinb | MSN |
| Annexin A1b\A2b\A5 | ANXA1\ANXA2\ANXA5 |
| Caldesmon | CALD1 |
| Endoplasmin | HSP90B1 |
| Tropomyosin α4 | TPM4 |
| Proteasome subunit beta type-2 | PSMB2 |
| Ubiquitin-40S ribosomal protein S27a | RPS27A |
| Cytoskeleton-associated protein 4 | CKAP4 |
| Thy-1 membrane glycoproteinb | THY1 |
| Protein S100-A6\-A11\-A10 | S100A6\S100A11\S100A10 |
a Previously reported interactions of Cx43.
b Constituent part of the plasma membrane.