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. Author manuscript; available in PMC: 2016 Oct 9.
Published in final edited form as: J Mol Biol. 2015 Aug 21;427(20):3285–3299. doi: 10.1016/j.jmb.2015.08.013

Figure 1. Co-expression and purification of P22 S:L-terminase complex.

Figure 1

(A) Domain organization of P22 S- and L-terminase subunits. (B) Chromatogram of the S:L-terminase complex purified in the presence of mild detergent, magnesium chloride and glycerol. The S:L-terminase complex was separated on a Superdex 200 gel filtration column after o/n digestion with PreScission Protease. Fractions corresponding to the eluted peaks were analyzed by SDS-PAGE (bottom gel) revealing pure S- and L-terminase subunits and free MBP.