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. Author manuscript; available in PMC: 2016 Oct 9.
Published in final edited form as: J Mol Biol. 2015 Aug 21;427(20):3285–3299. doi: 10.1016/j.jmb.2015.08.013

Figure 4. The N-terminus of L-terminase ATPase domain binds specifically to the LBD.

Figure 4

(A) Pull-down assay on glutathione beads coupled to GST-LBD reveals L-terminase ATPase domain is sufficient for binding to S-terminase. (B) Ribbon diagram of a homology model of P22 L-terminase (generated using i-TASSER [67]) with S-terminase Binding Domain (SBD), ATPase and nuclease domains colored in green, red and yellow, respectively. (C) Co-expression of FL- and Δ58-L-terminase with GST-LBD in bacteria followed by pull-down on glutathione beads and SDS-PAGE analysis reveals the SBD is essential for association with the LBD.