Figure 2.

Phylogenetic analysis of 2-oxoglutarate-Fe²⁺ dioxygenases involved in phenolic secondary metabolism, including enzymes from flavonoid biosynthesis. The phylogenetic tree was constructed with the aligned protein sequences with MEGA (version 5.10; Tamura et al., 2011) using the neighbor-joining method with bootstrap (10,000 replicates). The protein sequences were aligned using ClustalW implemented in MEGA 5.10. Different oxoglutarate-Fe²⁺ dioxygenases are clustered in circles based on their major demonstrated activities.