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. 2015 Aug 12;169(2):1090–1107. doi: 10.1104/pp.15.00515

Figure 4.

Figure 4.

In vitro ZmFNSI-1 activity assayed with naringenin as a substrate. A, LC-MS analysis of purified His-6-ZmFNSI-1 activity. The reaction product generated a molecular ion of mass-to-charge ratio (m/z) = 271 corresponding to apigenin (positive ion chromatogram). A negative control (without protein) did not show the production of any product. B, MS/MS fragmentation profile of the product of the purified His-6-ZmFNSI-1 activity assay. C, Apigenin standard was used as a control. Its MS/MS fragmentation profile corresponds to that of the His-6-ZmFNSI-1 reaction product, which is shown in the inset.