Figure 1.

tem1 tem2 mutant plants are early flowering at 16°C but still sensitive to changes in ambient growth temperature. Flowering time was measured as the number of total leaves produced at flowering (A) and the number of days to flowering (B) for wild-type (Col-0) plants and tem1 tem2 and svp mutants grown under LD conditions at 22°C (black) or 16°C (white). Data are reported as mean ± sem of three independent experiments (each dot plot represents an independent experiment; red circles indicate experiments performed at 22°C, and blue squares indicate experiments performed at 16°C). A minimum of 12 plants per genotype and experimental condition was analyzed in each independent experiment. The numbers below the bars denote the leaf number ratio (16°C/22°C). For more details, see Supplemental Table S1. C and D, Reverse transcription followed by quantitative real-time PCR (RT-qPCR) analysis of FT expression in wild-type (black triangles), tem1 tem2 (gray squares), and svp (gray circles) plants in 9-d-old seedlings grown under LD conditions at 22°C (C) or 16°C (D). Samples were collected over a 24-h period. The dark period is denoted by the black bar. Two independent experiments gave similar results (Supplemental Fig. S1), and one was chosen as representative. RNA levels were normalized to UBQ10. Error bars show sd of three technical replicates.