Figure 4.

Binding of TEM2 protein to the FT and TSF promoters at 16°C. ChIP assay of binding of TEM1-HA and TEM2-HA proteins to the RAV motifs in the FT (A) and TSF (B) promoters. Fragments containing the canonical RAV binding site for FT, a putative RAV binding site for TSF, and noncontaining RAV binding sequences (used as negative controls [NCs]) were analyzed by ChIP using 9-d-old 35S::TEM1 and 35S::TEM2 plants carrying an HA tag. Precipitated chromatin was used as a template in qPCR. Immunoprecipitated DNA enrichment is presented as a percentage of input DNA. Two (FT) or three (TSF) independent experiments gave similar results (Supplemental Fig. S7), and one was chosen as representative. Error bars show sd of three technical replicates. Schematic diagrams of the FT and TSF promoters are shown below graphs. Arrows indicate fragments amplified by qPCR after ChIP. WT, Wild type.