Genetic interaction between tem and svp mutants. A, Flowering time measured as the number of total leaves produced at flowering for the wild type (Col-0), tem mutants, and svp tem double and triple mutants grown under LD conditions at 22°C or 16°C. Data are reported as mean ± sem of two independent experiments (each dot plot represents an independent experiment; red circles indicate experiments performed at 22°C, and blue squares indicate experiments performed at 16°C). A minimum of 10 plants per genotype and experimental condition was analyzed in each independent experiment. The numbers below the bars denote the leaf number ratio (16°C/22°C). For more details, see Supplemental Table S2. B and C, Photographs of plants used in flowering time analysis grown for 24 d at 22°C (B) and 32 d at 16°C (C).