Figure 3.

Subcellular localization of GFP fused to different deletions of βCA1 in transiently transformed tobacco epidermes. CA1-GFP is the full-length βCA1 protein fused to GFP that localizes to chloroplasts. CA1ΔCP-GFP is the GFP-fused βCA1 protein with the first 65 amino acids truncated, which includes a sequence from the chloroplast signal peptide. CA1CP-GFP represents the first 65 amino acids only containing chloroplast signal peptide sequence of βCA1 fused to GFP that expresses in chloroplasts. FLAGELLIN-SENSING2 (FLS2) was used as a control plasma membrane protein. A, D, G, and J, GFP. B, E, H, and K, Chloroplast autofluorescence. C, F, I, and L, Merged images.