Figure 4.
Plasma membrane targeting of βCA4 restores CO2 responsiveness when expressed in ca1ca4 mutant guard cells. A, Confocal images showing CA4-YFP localization at the plasma membrane of ca1ca4 guard cells of line CA4YFP-68. Aa, YFP; Ab, red chlorophyll autofluorescence; Ac, merged image. B to E, Time-resolved stomatal conductance in two independent CA4-YFP-expressing ca1ca4 lines and control plants. Absolute stomatal conductance data are shown in B and D, and their corresponding relative stomatal conductance data are shown in C and E. n = 4 for each genotype. Data represent mean ± sem. WT, Wild type.